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PI
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WO
Studiejaar 6
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GLP
Use the following as your name:
PhD
PI
Student
Slide 1 - Diapositive
pH meter:
How often do you have to calibrate the pH meter?
A
Once a year
B
Once a month
C
Once a week
D
Every experiment
Slide 2 - Quiz
pH Meter:
Which is the optimal buffer we can use for proteins?
A
Tris
B
HEPES
C
Phosphate
D
Depends on your protein
Slide 3 - Quiz
Nitril Gloves:
How many gloves do you wear while moving in the lab?
A
0
B
1
C
2
D
Depends on your experiment
Slide 4 - Quiz
Weighing equipment:
Do we need to balance each
balance?
A
No
B
Yes
Slide 5 - Quiz
Weighing equipment:
After using a balance which cleaning steps do you need to perform?
A
Clean under/around the balance
B
Clean weighing tray
C
Clean used spatulas
D
Leave it for the next person
Slide 6 - Quiz
Ultra centrifugation:
Which balance do you use for balancing your tubes?
A
B
C
Slide 7 - Quiz
Pipetting:
For accurate pipetting, do you have to pre-rinse your pipet tips and how many times?
A
No
B
Yes, 1 time
C
Yes, 3 times
D
Yes, 5 times
Slide 8 - Quiz
Using Enzymes:
How do you transport or use restriction enzymes always when in the lab?
A
On ice
B
In the -20C box
C
pipet next to the freezer
D
take it to your bench and return asap
Slide 9 - Quiz
Lab coats:
When do you need to where your lab coat?
A
Always when on the lab
B
Only when you work longer than 5 minutes
C
Only when you work with dangerous chemicals
D
Never
Slide 10 - Quiz
SDS page gels:
What is the sensitivity of a standard coomassie staining
A
30-100 µg protein
B
3 - 10 µg protein
C
0.3- 1 µg protein
D
0.03 µg - 0.1 µg protein
Slide 11 - Quiz
SDS page gels:
For coomassie staining your gel which of the following procedures is correct.
A
15 minute wash in water, >1 hour in staining, Destain at least 1 hour
B
>1 hour in staining, Destain at least 1 hour
C
15 minute wash in water, O/N in staining, Destain O/N
D
O/N in staining, Destain O/N
Slide 12 - Quiz
DNA gels:
Which dilution can we use the Gel-red staining?
A
1:5000
B
1:10000
C
1:30000
D
1:100000
Slide 13 - Quiz
Fume hood:
How do we leave the fume hood window after working there?
A
Window open
B
Windows closed
C
Window closed, Fume hood off
D
Window open, Fume hood off
Slide 14 - Quiz
Equipment:
The equipment I used gave errors or made weird noises, what to do next?
A
Leave for the next person to figure out
B
Contact one of the technicians
C
Try to solve it yourself
Slide 15 - Quiz
When will the technicians be able to finish the applepie experiment?
A
Never
B
Within one week
C
Within one month
D
Within one year
Slide 16 - Quiz
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