GLP

• Onderwijswetenschappen
• WO
• Studiejaar 6

GLP

Use the following as your name:

PhD

PI

Student

pH meter:
How often do you have to calibrate the pH meter?

1. Once a year
2. Once a month
3. Once a week
4. Every experiment

pH Meter:
Which is the optimal buffer we can use for proteins?

1. Tris
2. HEPES
3. Phosphate
4. Depends on your protein

Nitril Gloves:
How many gloves do you wear while moving in the lab?

1. 0
2. 1
3. 2
4. Depends on your experiment

Weighing equipment:
Do we need to balance each
balance?

1. No
2. Yes

Weighing equipment:
After using a balance which cleaning steps do you need to perform?

1. Clean under/around the balance
2. Clean weighing tray
3. Clean used spatulas
4. Leave it for the next person

Ultra centrifugation:
Which balance do you use for balancing your tubes?

1. 
2. 
3. 

Pipetting:
For accurate pipetting, do you have to pre-rinse your pipet tips and how many times?

1. No
2. Yes, 1 time
3. Yes, 3 times
4. Yes, 5 times

Using Enzymes:
How do you transport or use restriction enzymes always when in the lab?

1. On ice
2. In the -20C box
3. pipet next to the freezer
4. take it to your bench and return asap

Lab coats:
When do you need to where your lab coat?

1. Always when on the lab
2. Only when you work longer than 5 minutes
3. Only when you work with dangerous chemicals
4. Never

SDS page gels:
What is the sensitivity of a standard coomassie staining

1. 30-100 µg protein
2. 3 - 10 µg protein
3. 0.3- 1 µg protein
4. 0.03 µg - 0.1 µg protein

SDS page gels:
For coomassie staining your gel which of the following procedures is correct.

1. 15 minute wash in water, >1 hour in staining, Destain at least 1 hour
2. >1 hour in staining, Destain at least 1 hour
3. 15 minute wash in water, O/N in staining, Destain O/N
4. O/N in staining, Destain O/N

DNA gels:
Which dilution can we use the Gel-red staining?

1. 1:5000
2. 1:10000
3. 1:30000
4. 1:100000

Fume hood:
How do we leave the fume hood window after working there?

1. Window open
2. Windows closed
3. Window closed, Fume hood off
4. Window open, Fume hood off

Equipment:
The equipment I used gave errors or made weird noises, what to do next?

1. Leave for the next person to figure out
2. Contact one of the technicians
3. Try to solve it yourself

When will the technicians be able to finish the applepie experiment?

1. Never
2. Within one week
3. Within one month
4. Within one year