DNA Analysis in Fire Scenes

November 2017

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Nask / BiologieMiddelbare schoolvwoLeerjaar 6

This lesson contains 47 slides, with interactive quizzes, text slides and 1 video.

Lesson duration is: 60 min

Items in this lesson

November 2017

Slide 1 - Slide

DNA Analysis in Fire Scenes

Milou Hekhuizen, MSc

Lecturer Forensic Laboratory Science & IT project manager

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3 locations

Breda, Tilburg, Den Bosch

Faculty of Technology of Health and Environment

1500 students

4 bachelor programs:

(incl. Chemistry, Biology and Forensic Laboratory Research)

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How to identify a person?

Slide 6 - Mind map

Can you identify this person?

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Could you identify the person you saw in the image?

Yes, most likely

Yes, definitely

I don't know

Slide 8 - Quiz

DNA analysis

- DNA structure

- Genomic DNA

- STR analysis

- mtDNA

-Y-DNA

- Discovery & identification of human remains

- From scene to trial

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Genomic DNA

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DNA strand

3 billion nucleotides

- Guanine

- Cytosine

-Thymine

-Adenine

0.1- 0.4% difference

(99.6-99.9% the same)

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Could we use genomic DNA to identify this person?

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Could we identify this person using genomic DNA?

Yes

Maybe

Slide 15 - Quiz

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STR analysis

Set of STR's =

unique combination

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Polymerase Chain Reaction (PCR)

- Copy specific pieces of DNA

* Make use of copy mechanisms of bacteria

- Exponential amplification

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Slide 19 - Video

Fluorescent label

Inherit 2 chromosomes

same STR --> different lengths

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DNA profile

NL: 12 loci

7 loci by European standards

Treaty of Prüm
matching rules

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Why mtDNA?

- many mitochondria

- maternally inherited

- small piece of DNA

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Drill or grind

Collect powder

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HV1 & HV2

Cambridge reference sequence

Single nucleotides

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Cambridge reference

List variations

Check variations with

references

Polymorphisms

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Y-chromosomal DNA

Paternally inherited

Passed on unchanged

(but with gradual

accumulation of mutations)

STR analysis

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Example use of Y-DNA

Marianne Vaatstra (1999)

Traces of blood and semen found

DNA profilling in 2012

Jasper S --> brother's DNA was tested

Marianne Vaatstra

Milica van Doorn

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Fire scenes

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What are the risks in the investigation of a fire scene?

Slide 34 - Mind map

Risks & difficulties

- Fire debris

- Discovery & identification human remains

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Calcined bone

- Brittle

- Grey-ish color

- Fragmented pieces

- >250 degrees Celcius: no amplification possible

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Any questions?

Slide 43 - Open question

Thank you for your attention and participation. What did you think about this lecture?

Slide 44 - Open question

More info about the minor or internships?

Send an email to Milou Hekhuizen:

mjd.hekhuizen@avans.nl

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Visualization

- Fluorescent labels/dye

- Within amplified piece of DNA

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Sequencing

- To find genes

- Human genome project

- Too much for personal identification (in the case of a crime)

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DNA Analysis in Fire Scenes

Items in this lesson

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Slide 6 - Mind map

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Slide 19 - Video

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Slide 34 - Mind map

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