3.5 (3) Introduction to cloning

• Biologie
• Middelbare school
• vwo
• Leerjaar 5

3.5 Cloning

Guiding Questions

To what extent is it acceptable to clone animals or humans?

What exactly is cloning?

How can we make a clone?

Aims:

- describe the DNA structure and how it suggests a method for replication

- describe the replication process using 'replication fork' & enzymes

- outline functions of coding and non-coding DNA

- outline base sequencing and DNA profiling

- describe findings: Herschy and Chase, Rosalind Franklin experiments

- describe nucleosomes within DNA

lesson outline (60min)

- Activity 1 Human Cloning is it possible or morally acceptable? Watch the video on the next slides and take notes using the tasksheet here

- Activity 2: Cloning ethics and nuclear transfer? Watch the video on the next slide and take the Cloning a mouse Activity simulation using flashplayer. Record notes using this tasksheet.

- Activity 3: A video Tour of Cloning (Watch Bill Nye - 25min!) and take notes using this tasksheet.

Debate about the ethics involved in cloning

Guiding questions

What are the issues around the production and use of stem cells for therapeutic cloning?

Where do I stand in the decision about the ethical issues of using stem cells for therapeutic treatments?

Therapeutic cloning is the creation of an embryo to supply embryonic stem cells for medical use.

Reproductive cloning: Dolly the sheep

Outline a technique for cloning using differentiated animal cells.
    1.Differentiated cell from donor sheep udder was collected and cultured; nucleus removed
    2.Unfertilized egg collected from another sheep; nucleus removed
    3.Using a zap of electrical current, the egg cell and the nucleus from the cultured somatic cell were fused together
    4.New cell developed in vitro and started to form an embryo
    5.Embryo placed in the womb of a surrogate mother sheep (Actually over 200 surrogate mothers )
    6.Embryo developed normally
    7.Dolly was born and presented as a clone of the original donor sheep
    8. This is known as reproductive cloning

"I think the production and use of stem cells poses more dangers than potential benefits."

Think for 1 minute

Breakout room 1: I agree

Breakoutroom 2: I disagree

Build arguments and present in debate.

The end of the lesson