DNA Lesson 3 Transcription

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• Middelbare school
• vwo
• Leerjaar 4

DNA Lesson 3 Transcription

Learning Goals Lesson 3 

p. 53/54

• You can describe the process of transcription
• You know why transcription takes place
• You know where transcription takes place

• Key words Lesson 3 - transcription

• RNA
• Ribose
• RNA-polymerase
• Uracil
• transcription
• mRNA
• rRNA
• tRNA
• promotor
• transcriptionfactor

template strand

matrix strand

coding strand

RNA - RiboNucleicAcid - Ribonucleïnezuur

RNA is build from  4 different building blocks (nucleotides); A,C,U,G

Every nucleotide is made of

3 parts:

1. phosphate

2. ribose (sugar)

3. nucleic acid 

Engels                                  Nederlands

nucleotide DNA vs nucleotide RNA

1. phosphate

2. ribose (sugar)

3. nucleic acid U instead of T

1. phosphate

2. desoxyribose (sugar)

3. nucleic acid T

Enzyme:                     RNA polymerase.                     

Location.                   Nucleus                                        Nucleus.                             Ribosome

Process                                    Transcription                                                                 Translation (next lesson)

Protein

Molecule

Building blocks

Nucleotides

Nucleotides

Amino acids

20 different kinds, glutamine, proline, leucine, etc.

      mRNA

Transcription

DNA is strictly rolled up on histons so RNA can not 

transcribe the DNA

DNA has loosened up so transcription of the gene can take place

Template or matrix DNA is copied to  mRNA

1. The template or matrix strand of DNA is transcripted by RNA polymerase to one strand of pre mRNA. 

2. Introns are cut out and exons are pasted together* (' RNA processing'). 3. The mRNA is transported out of the nucleus towards the ribosomes.

1.

2.

3.

* V5 lesson material

The following DNA strand is used as a template for transcription:
'3 CGTAAGCGGCT '5

Which of the following mRNA strands will be produced?

1. '5 AGCCGCUUACG '3
2. '5 GCAUUCGCCGA '3
3. '5 CGUAAGCGGCU '3
4. '5 UCGGCGAAUGC '3

Part of the coding strand of a double helix DNA strand has the nucleotide sequence: CGGATACGGTTA.
What is the sequence of the nucleotides in the mRNA-molecule that is synthetised based on this D(S)NA sequence?

1. GCCTATGCCAAT
2. GCCUAUGCCAAU
3. CGGAUACGGUUA
4. UAACCGCACCCG

Learning Goals Lesson 3

• You can describe the process of transcription
• You know why transcription takes place
• You know where transcription takes place

Homework

(For next week)

• Worksheet lesson 3 DNA transcription
• Make question 1 - 4 out of your book (page 57)