Experiment DNA extraction

• Biologie
• Middelbare school
• havo
• Leerjaar 4

 DNA

practicum

Answers 

1. 21(n) x 10 (decaploid) = 210 chromosomes
2. Extraction = separation method in which one or more components are extracted from a mixture using a solvent
3. Nucleus (also mitochondria and chloroplasts)
4. Cell wall/cell membrane and nuclear membrane
5. 
   

Answers 

6. Phosphates are hydrophilic, the two fatty acid tails are hydrophobic

7. Liquid soap

8. (not a question)

9. Histones are proteins made out of amino-acids

10. The liquid

DNA extraction

We will work in the lab so

we follow lab rules! 

fu

rules - lab experiments

• zero-tolerance : misbehave ==> 201
• Wear your safety equipment (glasses/coat/hair tied back)
• NO phones

• NO eating or drinking
• NO running , pushing, throwing or yelling
• DON'T touch materials you don't need
• Respect each other and the materials!!! 
•  Question? Ask TOA or teacher

What will we do?

1. Prepare the extraction solution water + soap + salt
   
2. To extract: separate the DNA from the strawberry cells
   
3. Precipitate ("neerslaan"): cause (a substance) to be deposited in solid form from a solution
   
4. Clean up!!!

What will we do? Tips!

- Add the soap to the beaker!!

Remember the names? 

Soap

Let's get to work! 

Bring:

pen/pencil

Good luck! 

You only have 30 minutes!!! 

Follow up DNA extraction 

What did we do?

Why did we add salt? 

Why did we add soap/detergent? 

Why did we add alcohol?

Salt: Using salt helps eliminate the histones, proteins around which DNA is wrapped.

Soap/detergent: The detergent destroys the membrane of the cell and the nucleus, allowing the DNA to come out

Alcohol: adding an ice-cold alcohol knocks DNA out of solution. This is because DNA is less soluble in an alcohol than in water.

Any questions?

Chapter Cells? 

ChapterDNA ?